WebI. t:JIrR01JJCl'tOI. • • • • • • • • • • .. .. • • • •• 1 . hrp ••• of . the . awctr .thod . aDd WebFeb 11, 2024 · The plasmid pHH21-SC09NS F-Luc, for the expression of a viral RNA-like firefly luciferase gene under the control of the human RNA polymerase I promoter, has …
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WebFeb 1, 2024 · After 24 h, cells were co-transfected with the four protein expression plasmids of the viral ribonucleoprotein (vRNP) complex (pCAGGS-PB2, pCAGGS-PB1, pCAGGS-PA, … WebPlasmid pHH21-BNA-Luc from Dr. Arindam Mondal's lab contains the insert Reporter RNA (RNA encoding firefly luciferase under the viral promoter of segment 6 of Influenza … mu2020 アーカイブ
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WebFeb 18, 2024 · Here, using RNAi library screening based on cellular transcription profiling, we found that the cyclin-dependent kinase inhibitor p21 (also known as p21 Waf1/Cip1) was … WebSep 2, 2024 · The construction of pHH21-SC09NS F-Luc, which was used to produce an influenza vRNA-like luciferase reporter, and an ISRE-Luc reporter has been previously described [20, 41]. The ORFs of human IMP α1, IMP α3, IMP α5, IMP α7, and IMP β1 with a Flag tag at the N-terminus were cloned into a pCAGGS vector. WebRT-qPCR analysis showed that the levels of all three species of viral RNA (i.e., vRNA, cRNA, and mRNA) were dramatically increased in A549 cells infected with the PG/S1421 (H7N9) mutant bearing the... mu/sc変換アダプタ